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The laboratory tests were done without prior knowledge of the results of the rapid tests.Evaluation Nike Blazer Red UK of small bowel biopsiesBiopsy samples were taken by Watson capsule or during upper gastrointestinal endoscopy from the distal part of the duodenum. The ratio of the villous height to the crypt depth was calculated17 by evaluators who were blinded for the nature Nike Blazer Mid Mens Sale of the samples. A ratio of less than one was considered to be villous atrophy with crypt hyperplasia and indicative of untreated coeliac disease (Marsh grade III lesion).6 Frozen sections were used to investigate numbers of CD3 positive and positive intraepithelial lymphocytes and in situ deposition of antibodies to transglutaminase. 4 18Clinical evaluationWe determined the children's weight and height centiles using reference values for Hungarian children19 Nike Blazer Mid Womens Sale and recorded any symptoms. We used standard laboratory methods to determine blood count and to measure serum iron, transferrin, and ferritin. HLA DQ alleles were determined by polymerase chain reaction (GenoVision SSP kit; Qiagen Vertriebs GmbH) in children with positive antibody tests. Parents of newly detected patients with coeliac disease and sex matched controls with negative antibody results answered a generic child health questionnaire, which had previously been validated in Hungarian children.
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