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coeliac disease by means of a rapid antibody test performed by local healthcare workers in primary care.MethodsSubjects and screening procedureWe screened 6 year old children in Jsz Nagykun Szolnok County, Hungary, which Mens Nike Blazer White
has a total of 413174 inhabitants. District nurses were asked to screen all children in their care born between 1 June 1998 and 31 May 1999, who were due to start school in 2005. These children had a preschool physical examination with weight and height measurements during the spring of 2005 at their primary care centre.
Nurses measured antibodies to transglutaminase on site using 10 l of whole blood from a finger prick and a test Nike Blazer Low Womens Shoes
that gives results in 5 10 minutes. Children with positive results on this rapid test were referred directly for small bowel biopsy to the paediatric gastroenterology unit of Hetnyi Gza County Hospital, Szolnok, Hungary.At the same time, the nurses collected 80 l of blood for the laboratory determination of IgA antibodies to endomysium and transglutaminase in plasma (the reference tests).
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of the samples. A ratio of less than one was considered to be villous atrophy with crypt hyperplasia and indicative of untreated coeliac disease (Marsh grade III lesion).6 Frozen sections were used to investigate numbers of CD3 positive and positive intraepithelial lymphocytes and in situ deposition of antibodies to transglutaminase.
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and recorded any symptoms. We used standard laboratory methods to determine blood count and to measure serum iron, transferrin, and ferritin. HLA DQ alleles were determined by polymerase chain reaction (GenoVision SSP kit; Qiagen Vertriebs GmbH) in children with positive antibody tests. Parents of newly detected patients with coeliac disease and sex matched controls with negative antibody results answered a generic child health questionnaire, which had previously been validated in Hungarian children.
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